Abstract

Natural history collections are the physical repositories of our knowledge on species, the entities of biodiversity. Making this knowledge accessible to society – through, for example, digitisation or the construction of a validated, global DNA barcode library – is of crucial importance. To this end, we developed and streamlined a workflow for ‘museum harvesting’ of authoritatively identified Diptera specimens from the Smithsonian Institution’s National Museum of Natural History. Our detailed workflow includes both on-site and off-site processing through specimen selection, labelling, imaging, tissue sampling, databasing and DNA barcoding. This approach was tested by harvesting and DNA barcoding 941 voucher specimens, representing 32 families, 819 genera and 695 identified species collected from 100 countries. We recovered 867 sequences (> 0 base pairs) with a sequencing success of 88.8% (727 of 819 sequenced genera gained a barcode > 300 base pairs). While Sanger-based methods were more effective for recently-collected specimens, the methods employing next-generation sequencing recovered barcodes for specimens over a century old. The utility of the newly-generated reference barcodes is demonstrated by the subsequent taxonomic assignment of nearly 5000 specimen records in the Barcode of Life Data Systems.

Addeddate

2024-09-09 16:45:42

Bhl_virtual_titleid

210877

Bhl_virtual_volume

v.11 (2023)

Call number

10_3897_BDJ_11_e100677

Call-number

10_3897_BDJ_11_e100677

Foldoutcount

0

Genre

article

Identifier

workflowexpandi11leve

Identifier-ark

ark:/13960/s2s1ptjhgh1

Identifier-bib

10_3897_BDJ_11_e100677

Identifier-doi

10.3897/BDJ.11.e100677

Identifier-elocator

e100677

Ocr

tesseract 5.3.0-6-g76ae

Ocr_detected_lang

en

Ocr_detected_lang_conf

1.0000

Ocr_detected_script

Latin

Ocr_detected_script_conf

1.0000

Ocr_module_version

0.0.21

Ocr_parameters

-l eng

Page_number_confidence

88

Page_number_module_version

1.0.5

Page_range

1-20

Pages

20

Pdf_degraded

invalid-jp2-headers

Pdf_module_version

0.0.25

Possible copyright status

In copyright. Digitized with the permission of the rights holder.

Ppi

300

Source

Biodiversity Data Journal 11

Year

2023

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